Thursday, January 17, 2008

Shirdi Sai-man makea life in test tubes!!!

After lecturing and studying about the Human genome and attended the sessions my mind was reluctant to go into any other thing...So fascinating the life and science’s.I happen to read The Book”DNA The secret of life” by james D.Watson with Andrew Berry in that book under the chapter playing god: Customised DNA molecules....I happen to read an interesting thing as follows..
“Aurther kornberg was the first to“make life” in a test tube. In 1950’s as we have seen, he discovered DNA polymerase, the enzyme that replicates DNA though the formation of a complementary copy from an unzipped “parent” strand. Later he would work with the pair of viral DNA, he was ultimately able to induce the application of all the virus’s 5300 base pairs of DNA but the product was not alive. Though identical in DNA sequence to it’s parent, it was biologically inert. Something was missing. The missing ingredient would remain a mystery till 1967.When Martin Gellert at the national Institute of health and Bob Lehmann at standford simultaneously identified it. This enzyme as “ligase” Ligase made it possible to Glue the ends of the DNA molecules together...
Kornberg could replicate the viral DNA using DNA polymerase and by adding ligase, join the two ends together so that the entire molecule formed a continuous loop, just as it did in the original virus, this artificial viral DNA behaved like a original one as exactly, the virus normally multiplies in E.Coli and kornbergs test-tube DNA did that. using just a couple of enzymes, some basic chemical ingredients, and viral DNA from which to make the copy Koernberg had made a biologically active molecule. The media reported he had created life in a test tube...”
My.... it is now 2.30 nights I felt it is better to go to bed. Meanwhile I have totally left the night aarti about my sai unsung today
My sairam is great he will not take it otherwise He knows ultimately I am his child

Aum sairam.

1 comment:

Raghav said...

i did not get the connection between your life science project & Sai Baba!
wht r u going to connect?????

waiting for more postings in this blog..........